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. 2009 Jun 9;4(6):e5842. doi: 10.1371/journal.pone.0005842

Figure 1. SAA3 but not SAA1/2 mRNA expression is increased in mouse adipose tissue in the presence of microbiota.

Figure 1

(A) qRT-PCR analysis of SAA3 mRNA expression levels in epididymal adipose tissue of germ-free (GF) and conventionally raised (CONV-R) mice (n = 10 mice per group). (B) qRT-PCR analysis of SAA1/2 mRNA expression levels in epididymal adipose tissue of germ-free (GF) and conventionally raised (CONV-R) mice (n = 10 mice per group). Data are means±SEM. ***P<0.001; Student's t-test. (C) Representative immunostaining of SAA3 (green) and bis-benzimide-labeled nuclei (blue) in CONV-R mouse epidiymal adipose tissue. (D) Primary antibody omitted. Scale bars = 200 µm.