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. 2009 Jun 8;4(6):e5833. doi: 10.1371/journal.pone.0005833

Figure 1. Crossing scheme to create an MLA mosquito strain.

Figure 1

A: Each transgenic line (A–D) is outcrossed to one of two wild-type backgrounds (denoted by colors). The final 8 outcrossed transgenic lines are made homozygous. B: The 8 outcrossed transgenic lines are specifically crossed and offspring of the correct genotype selected each generation to construct the MLA strain. Correct genotyping of offspring can potentially be automated in a high-throughput manner (see text and Figure 8). C: Hypothetical locations of each transgenic locus so that all 4 loci assort independently.