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Changes in transgene frequency with a 5% discontinuous release every 10 generations of mosquitoes homozygous for a transgene at 4 unlinked loci (8 copies/genome). Releases are halted after the fourth release. If transgenes are costly, selection eliminates the construct from the population. Thick solid line: no fitness cost (F = 1.0), thin solid line: 1% fitness cost/insert (F = 0.99), thin dotted line: 5% fitness cost/insert (F = 0.95).
