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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Dev Cell. 2009 Apr;16(4):528–538. doi: 10.1016/j.devcel.2009.02.009

Figure 5. SRp38 specifically activates splicing of a substrate containing triadin 1-specific exon 9 RNA.

Figure 5

(A) SRp38 activates splicing of a substrate containing triadin 1-specific E9. In vitro splicing was performed in Hela S100 extract using E9, E9 mutant or E10 containing substrates (β-E9, β-E9 mutant or β-E10) with indicated additions. SRp38 splicing activity in S100 requires a nuclear fraction (NF40–60) prepared by ammonium sulfate precipitation. (B) Splicing of β-E9 in vitro in the presence of unlabelled competitors. Competitors were used at 3 or 9-fold molar excess over the pre-mRNA.