Table 2.

Quantitative analysis of CACNA1C splice variants in human heart left ventricle (n = 65) using fluorescently labeled primers or real-time PCR (labeled with #)

Locus	Splice variants	65 samples measured individually		Pooled sample from 65 heartsa	Pooled sample from five normal heartsb	Functional consequencea(ref)
		Mean ± SD (%)	Range % (min to max)
1#	E1a-E2	70 ± 7	47–87	67	71	Activated by PKC [15]
	E1-E2	30 ± 7	13–53	33	29	Inhibited by PKC [15]
2	E2-E3	99.9 ± 0.1	99–100	100	100
	E2-E3 + 4nt	0.04 ± 0.10	0–1	0	0	Frame shift
3	E6-E7	50 ± 3	40–59	52	53
	E6-E7-12nt	50 ± 3	41–60	48	47
4a	E7-E8/E8a-E9-E10	99 ± 1	91–100	95	96
	E7-E9-E10	1 ± 1	0–9	3	3
	E7-E10	0	0	2	1
4b#	E7-E8	26 ± 12	13–91	25	15	Smooth muscle isoform, higher affinity for DHP [18]
	E7-E8a	74 ± 12	9–87	75	85	Cardiac isoform, lower affinity for DHP [18]
5	E9-E10	98 ± 1	94–99	97	96
	E9-E9*-E10	2 ± 1	0.6–6	3	4
6	E10-E11	99.0 ± 0.4	97–100	99	99
	E10-E10*-E11	1.0 ± 0.5	0–3	1	1
7	E14-E15	75 ± 4	60–83	75	78
	E14-E15-73nt	25 ± 4	17–40	25	22	Frame shift, hemi-channel [11]
8	E16-E17	99.0 ± 0.4	98–99	96	97
	E16-E17 + 9nt	1 ± 0.4	0.2–2.3	4	3
	E16-E17 + 12nt	0.2 ± 0.2	0–1	0.2	0.2
9a	E20-E21/22-E23	99.0 ± 0.6	97–100	98	98
	E20-E23	1.0 ± 0.6	0–2	2	2
9b#	E20-E21	91 ± 2	81–94	89	87
	E20-E22	9 ± 2	6–19	11	13	More sensitive to DHP [14]
10a	E30-E34	0.2 ± 0.4	0–2	0.3	0.2	Nonfunctional channel [11]
	E30-E33-E34	2 ± 1	1–7	1	0.6
	E30-E31-E32-E34	1 ± 0.2	0–1	0.5	0.5
	E30-E31-E32-6nt-E34	0.03 ± 0.06	0–0.3	0	0
	E30-E31-E32-E33-E34	1.0 ± 0.5	0.3–3	1.00	1
	E30-E31-E32-6nt-E33-E34	0.2 ± 0.2	0–1	0	0.2
	E30-E31/E32-E33-E34	48 ± 8	16–63	50	42	Reference isoform [11]
	E30-E32-6nt-E34	3 ± 1	1–6	3	2.9	A isoform, Change gating property [11]
	E30-E32-6nt-E33-E34	15 ± 2	11–26	10	20	B isoform,
	E30-E31/E32-E34	24 ± 6	9–41	34	32	C isoform
10b#	E30-E31	13 ± 6	5–33	20	19	Fetal isoform [19]
	E30-E32	87 ± 6	67–95	80	81	Adult isoform [19]
11	E40-E41	96 ± 1	93–98	93	92
	E40-E41 + 57nt	4 ± 1	2–7	7	8	Change gating property [13]
12	E44-E46	99 ± 3	90–100	99	98
	E44-E45*-E46	1 ± 3	0–9	1	2
	E44-E45-E46	0	0	0	0
	E44-E45*-187nt-E46	0	0	0	0

Data represent the percentage of each splice variant of the total transcripts in each locus. Data are the mean ± SD of 65 samples, each was measured twice. PKC, protein kinase C; DHP, dihydropyridine.

^aSame amounts of cDNA from 65 heart tissues were pooled, and splice variants measured twice and the average was shown.

^bSame amounts of total RNA from five normal heart left ventricles were pooled, then the cDNA synthesized and the splice variants measured. Data are the average of two cDNA preparations.