Table 2.
ELISA measurements of Aβ 40 and Aβ 42 after a three-step extraction from the anterior cortex of Tg2576 mice following 6 months of passive immunization with either control IgG or anti-Aβ antibody 2H6
| Aβ | Age (months) | Treatment | TBS [ng/mg (%)] | 2% SDS [μg/mg (%)] | 70% FA [ng/mg (%)] | Total [ng/mg (%)] |
|---|---|---|---|---|---|---|
| Aβ 40 | 14 | Control IgG | 0.7 ± 0.2 (2.0) | 26 ± 11 (65) | 6.8 ± 3.5 (17) | 33.5 (83) |
| 2H6 | 0.4 ± 0.1 (3.0) | 6.0 ± 1.4 (43) | 4.1 ± 1.2 (29) | 10.5 (76) | ||
| 17 | Control IgG | 1.7 ± 0.5 (1.0) | 135 ± 19 (60) | 21 ± 3.4 (9.0) | 157.7 (70) | |
| 2H6 | 1.0 ± 0.3 (1.0) | 33.0 ± 5.1 (42) | 18 ± 2.9 (23) | 52 (66) | ||
| Aβ 42 | 14 | Control IgG | 0.2 ± 0.03 (1.0) | 6.0 ± 1.9 (15) | 0.5 ± 0.2 (1.0) | 6.7 (17) |
| 2H6 | 0.1 ± 0.02 (1.0) | 3.0 ± 0.4 (22) | 0.3 ± 0.01 (2.0) | 3.4 (24) | ||
| 17 | Control IgG | 0.7 ± 0.2 (0.3) | 64 ± 10 (28) | 3.9 ± 0.6 (2.0) | 68.6 (30.3) | |
| 2H6 | 0.4 ± 0.2 (5.0) | 22 ± 10 (28) | 4.4 ± 1.1 (6.0) | 26.8 (34) |
Numbers in parentheses show the percentage of total Aβ in the fraction.