Tube 1.
| Component | Amount (μl) |
|---|---|
| Cy3 and Cy5 labeled and cleaned test and reference DNAs | ~160 (entire cleaned labeling reaction) |
| Human Cot1 DNA (1 μg μl −1) | 135 |
| 3 M NaAc pH 5.2 | 35 |
| 100% EtOH (cold) | 1,000 |
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