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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Nat Protoc. 2007;2(3):577–587. doi: 10.1038/nprot.2007.53

TABLE 1.

Setup for automated array hybridization (Step 33B(ix)).

Step Temperature (°C) Options
1 Wash 37 First: yes, channel: 1, runs: 1, wash time: 0:00:30, soak time: 0:00:00
2 Injection 37
3 Hybridization 37 Agitation frequency: medium, time: 0:45:00
4 Injection 37
5 Hybridization 37 Agitation frequency: medium, time: 21:00:00
6 Wash 37 First: no, channel: 1, runs: 15, wash time: 0:00:30, soak time: 0:00:30
7 Wash 54 First: no, channel: 2, runs: 5, wash time: 0:01:00, soak time: 0:02:00
8 Wash 23 First: no, channel: 1, runs: 10, wash time: 0:00:30, soak time: 0:00:30
9 Wash 23 First: no, channel: 3, runs: 1, wash time: 0:00:30, soak time: 0:00:00
10 Slide Drying 23 Time: 0:02:30, final manifold cleaning: yes, channel: 3

Liquid identification: channel 1: PBS/0.05% Tween 20, 2 mM cysteamine; channel 2: 0.1× SSC; channel 3: pure water.