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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: J Am Chem Soc. 2008 Dec 24;130(51):17384–17393. doi: 10.1021/ja804736t

Figure 2.

Figure 2

(a) Circular dichroism spectra of an equilibrium mixture of UBI-SDSn obtained by unfolding native UBI by dialysis against tris-glycine buffers containing concentrations of SDS from 0 to 10 mM. (b) Fractions (fk) of individual secondary structure component k contributing to the CD spectra of complexes of UBI and SDS [where k represents respectively α-helices (●), β-strands (■), turns (○), and random coils (+) secondary structure]. Values were averaged from the output results from the secondary structure estimation of three programs (CDSSTR, SELCON3, and CONTINLL; see Supporting Information for details). (c) Kinetics of hydrogen-deuterium exchange for complexes of UBI and SDS with up to 3.0 mM SDS. Hydrogen exchange is expressed in terms of the total number of hydrogens that are protected from exchange, determined from the average of six molecular ions at [SDS] = 0.0 (□), 0.4 (●), 1.4 (○), and 3.0 mM (■); hydrogen exchange was not measured above 3.0 mM SDS due to the quenching of electrospray ionization by the nonvolatile SDS.