Fig. 5.

Itk^−/− γδ T cells up-regulate costimulatory molecules involved in B cell help. (A) Thymocytes from WT and Itk^−/− mice were pooled and left nonstimulated (0 hours) or were stimulated with 10 μg/ml α-TCRδ for 24 hours. Nonstimulated and stimulated TCRδ⁺TCRβ⁻ T cells were then analyzed for the expression of ICOS, CD40L, and OX40. (B) WT and Itk^−/− thymocytes stimulated for 24 hours with 10 μg/ml α-TCRδ antibodies were stained for CD4, ICOS, CD40L, OX40, and NK1.1 expression.