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Copper damages iron-sulfur cluster dehydratases in the absence of oxygen. (A and B) W3110 (WT) and LEM33 (copA cueO cusCFBA) cultures were grown anaerobically in glucose/alanine medium to an OD500 of ≈0.1, and then challenged with 0 μM (open bars) or 4 μM CuSO4 (gray bars) for 30 min. (A) IPMI activity: copper-treated cells had IPMI activities below the detection limit (<15%). (B) Fumarase activity: the data are the average of 3 independent experiments, and the error bars represent SD.
