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Cu(I) damages the iron-sulfur cluster of fumarase A. (A and B) Fumarase A (4 μM) was challenged anaerobically at 27 °C with 0 μM (open squares), 10 μM (closed squares), 30 μM (closed circles), or 50 μM (closed diamonds) Cu(I) for 3 min. (A) Fumarase activity after challenge with Cu(I). (B) Time course of iron release from fumarase during copper challenge in A. The data are representative of 3 independent experiments.
