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. 2009 May 5;106(20):8368–8373. doi: 10.1073/pnas.0903392106

Fig. 5.

Fig. 5.

Anti-tumor effects of PU-H71 are associated with down-regulation of several Hsp90-regulated malignancy driving proteins. (A) Representative MDA-MB-468 tumors harvested at 50 days into treatment from the control (Upper) and the 75 mg/kg 3×week (Lower) arms at 12 h after the last administered dose, were extracted, fixed in formalin, and paraffin-embedded. Samples were immunostained for markers indicating tumor proliferation (p-HistoneH3), aggressiveness (Akt phosphorylation at Ser-473), and apoptosis (TUNEL). (B) Mice bearing MDA-MB-468 tumors were administered i.p. PU-H71 at the indicated doses and schedules for 2 weeks (n = 2 mice per dose and schedule). Mice were killed at 12 h after the last administered dose, and tumors were harvested. Protein extracts from tumors were subjected to western blot analysis (Upper Left), and changes in protein levels quantified by densitometry and data graphed (Lower Left). Representative tumors were fixed in formalin, paraffin-embedded, and immunostained for apoptosis (TUNEL) (Right).