Table 1.

Design of gene targeting for generating founder knock-out lines for selected polarity genes

Target gene	Target chromosome	Exons/mRNA isoforms	5′ + 3′ armsa (kb)	Targeted gDNA deletionb	Genomic deletion size (kb)	Protein deletion/full length (aa)
stardust	X	26/7	4.5 + 3.2	X: 8,129,169–8,134,146	4.977	741/2,020
lgl	second	9/6	5.2 + 3.1	2L: 21,725–9,743	11.982	1,161/1,161
DE-Cad (shotgun)	second	2/1	5.2 + 3.2	2R: 16,942,814–16,937,965	4.849	1,298/1,507
dArf6	second	3/5	4.5 + 3.1	2R: 11,210,875–11,213,032	2.157	175/175
CG31158	third	14/2	5.3 + 2.8	3R: 18,424,078–18,431,499	7.421	1,474/1,480
crumbs	third	13/2	5.2 + 2.9	3R: 20,130,302–20,140,245	9.943	2,109/2,189

^a5′+ 3′ arms: the lengths of 5′ and 3′ homologous arms in targeting construct.

^bAccording to Drosophila genome release 5.1 at www.flybase.org.