Fig. 2.

TβRIII alters the actin cytoskeletal organization of epithelial and cancer cells. (A–L) We plated 5–8 × 10⁴ of the indicated cell lines expressing the indicated proteins on coverslips and stained for actin by using rhodamine phalloidin. For transiently expressing TβRIII-IRES-GFP and control-GFP, cells were fixed and stained 48 h after infection and GFP-expressing cells were examined. Closed arrows indicate distinct lamellipodia in control cells and open arrows indicate rounded cells with multiple microspikes in TβRIII-expressing cells. Scale bar = 8.6 μm. (M and N) Subconfluent NOSE007 cells were infected with adenovirus expressing control shRNA NTCs (M) or shRNA-TβRIII (N). Cells were fixed and stained with actin-phalloidin-conjugated Alexa-488 72 h after infection and dsRed-expressing cells were examined. Open arrows indicate lamellipodia formation in shRNA-TβRIII cells. Scale bar = 21.6 μm.