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. 2009 Apr 30;106(20):8163–8168. doi: 10.1073/pnas.0903491106

Fig. 2.

Fig. 2.

RNase P cleavage in vitro of complexes formed by target mRNAs from the gyrA gene. EGS and PPMO-EGS directed cleavage of gyrA mRNA in vitro. Control lanes are: 1, gyrA mRNA alone; 2, gyrA mRNA with E. coli RNase P holoenzyme. An internally-labeled fragment of gyrA mRNA (10 nM) was incubated with E. coli RNase P holoenzyme (10 nM M1 RNA, 100 nM C5 protein) in the presence of increasing amounts (100 nM, 500 nM, 1 μM) of EGS241 (lanes 3–5), PPMO-EGS241 (lanes 6–8) or rEGS313 (lanes 9–11); lanes 12–14: gyrA mRNA with 1 μM EGS241, PPMO-EGS241, or prEGS313, respectively without RNase P. Reactions were incubated for 30 min at 37 °C and analyzed on 5% denaturing PAGE. Dots indicate the positions of cleavage products.