Table 3.
Inactivation of chloramphenicol resistance gene in E. coli in vivo [50 μM PPMO-EGS each; 4 hr after administration (LB broth-chloramphenicol (50 μM)]
| PPMO-EGS | Colony counts | Fraction of survival |
|---|---|---|
| Control | 4.1 × 105 | 1.0 |
| Scrambled | 4.0 × 105 | 0.98 |
| EGS 1 | 3.9 × 104 | 0.098 |
| EGS 2 | 6.5 × 103 | 0.016 |
| EGS 1 + 2 | 6.0 × 102 | 0.0015 |
Scrambled PPMO-EGS contains a sequence that has no relation to the targeted mRNA. At least 2 experiments were done, and averages are presented. PPMO-EGS concentrations are accurate within 10%. Naked RNA EGSs were not tested because they have little probability of entering the cells under test.