Table 2.

High concentrations of prostaglandin E₂ are present in the inflammatory exudates of COX-2 knockout mice

Mouse type	Control		Aspirin
	Air pouch prostaglandin E2, pmol/ml	No. of mice	Air pouch prostaglandin E2, pmol/ml	No. of mice
Wild type	2,016  ±  793	5	578  ±  83	3
NFκB knockout	2,879  ±  560	10	785  ±  37	5
COX-2 knockout	3,109	2	353	2

Data are presented as means ± SEM. Female C57/B16, 129 (wild type), NFκB (p105) knockout, and COX-2 knockout mice were treated with saline or aspirin (100 mg/kg) for 3 days by oral gavage during the induction of the air pouch. The pouches were injected with 2% (wt/vol) carrageenan, and 4 h later, the animals were killed, and the exudates were collected and kept frozen (−20°C) until the prostaglandin E₂ content was assayed by using a commercial ELISA kit. Each determination was carried out in triplicate.