Table 1.
List of genes used in this study
Symbol | Gene name | Ortholog | ToxoDB ID | Promoter length (bp) |
Gylcolysis | ||||
HK | Hexokinase | + | 57.m00001 | 900 |
G6PI | Glucose-6-phosphate-isomerase | + | 76.m00001 | 2,000 |
PFK | Phosphofructokinase | 49.m03242 | 2,000 | |
ALD | Aldolase | + | 46.m00002 | 1,370 |
TPI | Triose-phosphate-isomerase | + | 42.m00050 | 2,000 |
GAPDH | Glyceraldehydye-3-phosphate dehydrogenase | + | 80.m00003 | 2,000 |
PGK | Phosphoglycerate kinase | 641.m000193 | 2,000 | |
PGM | Phosphoglucomutase | 113.m00016 | 1,500 | |
ENO | Enolase* | + | 59.m03410 | 2,000 |
PyK | Pyruvate kinase | 55.m00007 | 1,500 | |
Nucleotide metabolism | ||||
AK | Adenosine kinase | + | 50.m00018 | 2,000 |
CTPS | Cytidine synthase | + | 129.m00261 | 2,000 |
DCDA | Deoxycytidine deaminase | + | 8.m00191 | 2,000 |
DHFR-TS | Dihydrofolate reducatase-thymidine synthase | 50.m00016 | 2,000 | |
GMPS | Guanidine monophosphate synthase | + | 44.m00023 | 2,000 |
RDPR | Ribonucelotide diphosphate reductase | 83.m00003 | 2,000 | |
UPRT | Uracil phosphoribosyl transferase* | 583.m00018 | 2,000 | |
AT | Adenosine transporter | 49.m00004 | 2,000 | |
Micronemal proteins | ||||
MIC1 | Microneme 1 | + | 80.m00012 | 1,500 |
MIC2 | Microneme 2 | + | 20.m00002 | 2,000 |
MIC3 | Microneme 3 | 641.m00002 | 2,000 | |
MIC4 | Microneme 4 | + | 25.m00006 | 2,000 |
MIC5 | Microneme 5 | + | 65.m00002 | 2,000 |
MIC6 | Microneme 6 | + | 38.m00003 | 2,000 |
MIC7 | Microneme 7 | 55.m00014 | 2,000 | |
MIC8 | Microneme 8* | 50.m00002 | 2,000 | |
MIC9 | Microneme 9 | 49.m03396 | 2,000 | |
MIC10 | Microneme 10 | + | 50.m00010 | 2,000 |
MIC11 | Microneme 11 | 20.m05914 | 2,000 | |
M2AP | Microneme-2-associated protein | 33.m00006 | 2,000 | |
Ribosomal proteins | ||||
RPS29 | Ribosomal protein S29 | 49.m03285 | 800 | |
RPS38 | Ribosomal protein S38 | 44.m04616 | 1,000 | |
RPS3 | Ribosomal protein S3 | 44.m04669 | 1,000 | |
RPS13 | Ribosomal protein S13 | 59.m03516 | 1,000 | |
RPL9 | Ribosomal protein L9* | 76.m00009 | 1,200 | |
RPS25 | Ribosomal protein S25 | 44.m00003 | 1,300 | |
RPS10 | Ribosomal protein S10 | 64.m00338 | 700 | |
RPL25 | Ribosomal protein L25 | 55.m00189 | 1,000 |
The list of genes and the lengths of their upstream regions that were used in the studies to identify regulatory motifs. A plus sign in the Ortholog column indicates that a corresponding ortholog in E. tenella was obtained and added to the search. Representative genes used in mutagenesis and expression analyses are denoted by an asterisk.