Table 2.
Biological pathways that are significantly enriched with E/HGTs
| Type | Functional category | Species group | Total enzymes | EGT | HGT |
| Map group | Lipid met | Ostreococcus | 64 | 10 (2.0*) | 3 |
| Map group | Lipid met | Plasmodium | 36 | 6 (3.2†) | - |
| Map group | Lipid met | Toxoplasma | 52 | 9 (3.7†) | - |
| Map | Bsyn of steroids | Diatoms | 22 | 7 (2.7†) | - |
| Map | Bsyn of steroids | Ostreococcus | 18 | 5 (3.5*) | - |
| Map | Bsyn of steroids | Theileria | 7 | 2 (7.0*) | - |
| Map | Fatty acid bsyn | Toxoplasma | 4 | 2 (10.7*) | - |
| Map | Fatty acid bsyn | Plasmodium | 5 | 2 (7.8*) | - |
| Map | Fatty acid met | Trypanosoma | 6 | 2 (10.1*) | - |
| Module | C5 isoprenoid bsyn, non-mevalonate | Diatoms | 7 | 4 (4.3†) | - |
| Module | C5 isoprenoid bsyn, non-mevalonate | Theileria | 6 | 2 (8.6*) | - |
| Module | C5 isoprenoid bsyn, non-mevalonate | Plasmodium | 7 | 2 (6.4*) | - |
| Module | Fatty acid bysn, elongation | Ostreococcus | 3 | 2 (6.6*) | - |
| Module | Fatty acid bysn, elongation | Plasmodium | 3 | 2 (15.0†) | - |
| Module | Fatty acid bysn, elongation | Toxoplasma | 3 | 2 (11.1†) | - |
| Map group | Carbohydrate met | Cryptosporidium | 65 | 8 (2.6†) | 2 |
| Map group | Carbohydrate met | Phytophthora | 195 | 22 (2.8†) | 5 |
| Map group | Carbohydrate met | Plasmodium | 82 | 10 (2.4†) | 2 |
| Map group | Carbohydrate met | Theileria | 61 | 6 (2.4*) | 1 |
| Map | Galactose met | Phytophthora | 8 | 2 (6.3*) | 1 |
| Map | Glycolysis/Gluconeogenesis | Phytophthora | 19 | 4 (5.3†) | 1 |
| Map | Glycolysis/Gluconeogenesis | Plasmodium | 13 | 3 (4.5*) | - |
| Map | Pentose phosphate pathway | Phytophthora | 12 | 3 (6.3†) | 1 |
| Map | Pyruvate met | Cryptosporidium | 8 | 2 (5.3*) | - |
| Map | Pyruvate met | Plasmodium | 13 | 3 (4.5*) | 1 |
| Map | Pentose and glucuronate interconversion | Leishmania | 6 | - | 3 (8.2†) |
| Map | Starch and sucrose met | Cryptosporidium | 11 | 2 | 2 (7.7*) |
| Map | Starch and sucrose met | Phytophthora | 22 | 5 (5.7†) | 1 |
| Module | Glycolysis | Phytophthora | 8 | 2 (7.0*) | - |
| Map group | Energy met | Ostreococcus | 54 | 9 (2.1*) | 2 |
| Map | Carbon fixation | Cryptosporidium | 8 | 2 (5.3*) | - |
| Map | Carbon fixation | Ostreococcus | 19 | 5 (3.3*) | - |
| Map | Nitrogen met | Toxoplasma | 11 | - | 2 (6.8*) |
| Map | Reductive carboxylate cycle | Leishmania | 6 | - | 2 (5.5*) |
| Map group | Other AAs | Leishmania | 32 | - | 5 (2.6*) |
| Map group | Other AAs | Phytophthora | 54 | 1 | 4 (3.6*) |
| Map | Arginine and proline met | Ostreococcus | 10 | - | 3 (5.3*) |
| Map | Glutamate met | Plasmodium | 15 | - | 2 (6.6*) |
| Map | Glutathione met | Leishmania | 10 | - | 3 (4.9*) |
| Map | Lysine bsyn | Toxoplasma | 6 | 1 | 2 (12.5†) |
| Map | Nicotinate and nicotinamide met | Leishmania | 4 | - | 2 (8.2*) |
| Module | Chorismate bsyn, phosphoenolpyruvate + erythrose-4P = > chorismate | Diatoms | 6 | 3 (3.8*) | - |
| Module | Histidine bysn, PRPP = > histidine | Ostreococcus | 5 | - | 2 (7.1*) |
| Module | Lysine bsyn, aspartate = > lysine | Toxoplasma | 5 | 1 | 2 (16.6†) |
| Map group | Cofactor and vitamins | Diatoms | 70 | 15 (1.8*) | 2 |
| Map group | Cofactor and vitamins | Leishmania | 28 | - | 5 (2.9*) |
| Map group | Cofactor and vitamins | Ostreococcus | 65 | 13 (2.5†) | 4 |
| Map | Biotin met | Saccharomyces | 4 | - | 2 (29.7†) |
| Map | Porphyrin and chlorophyll met | Diatoms | 20 | 12 (5.1†) | - |
| Map | Porphyrin and chlorophyll met | Leishmania | 4 | - | 2 (8.2*) |
| Map | Porphyrin and chlorophyll met | Ostreococcus | 21 | 12 (7.1†) | 2 |
| Map | Thiamine met | Diatoms | 3 | - | 2 (22.4†) |
| Module | Biotin bsyn, pimeloyl-CoA = > biotin | Saccharomyces | 3 | - | 2 (31.4†) |
| Module | Heme bsyn, glutamate = > protoheme/siroheme | Diatoms | 10 | 6 (4.5†) | - |
| Module | Heme bsyn, glutamate = > protoheme/siroheme | Leishmania | 3 | - | 2 (10.3*) |
| Module | Heme bsyn, glutamate = > protoheme/siroheme | Ostreococcus | 10 | 5 (4.9†) | 1 |
| Map group | Glycan bsyn | Phytophthora | 9 | 1 | 2 (10.9*) |
| Map | Lipopolysaccharide bsyn | Phytophthora | 5 | 1 | 2 (19.6†) |
| Map group | Xenobiotics biodegradation and met | Ostreococcus | 15 | - | 3 (3.5*) |
| Map | Aminoacyl-tRNA bsyn | Diatoms | 19 | 6 (2.7*) | 1 |
Significant over-representation of E/HGTs in biological pathways is shown on the following levels: Map group, KEGG map group; Map, KEGG map; Module, KEGG module. 'Total enzymes' is the number of enzymes in the species group within the defined category; 'EGT' and 'HGT' are counts of the number of transferred enzymes in the category followed in parentheses by the over-representation statistic for E/HGTs in that category (the proportion of E/HGTs for the category divided by the proportion of E/HGTs over all categories). Only statistically significant over-representation is shown and is indicated by asterisks (95% level) and dagger symbols (99% level). Significantly enriched pathways are not listed if the pathways contained only one E/HGT. The pathways are grouped by the KEGG map group they belong to in the following order: 'lipid metabolism', 'carbohydrate metabolism', 'energy metabolism', 'amino acid metabolism' and 'metabolism of other amino acids', 'metabolism of cofactors and vitamins', 'glycan biosynthesis and metabolism', 'xenobiotic biodegradation and metabolism' and 'amino-tRNA biosynthesis'. Abbreviations used in the pathway names: AA, amino acids; bsyn, biosynthesis; met, metabolism.