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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Cancer Res. 2009 Mar 31;69(8):3529–3536. doi: 10.1158/0008-5472.CAN-09-0067

Figure 5.

Figure 5

GCC signaling reduces hematogenous seeding of mouse lung. A, Representative inverted fluorescence microscope fields of MitoTracker-stained wild type T84 cells and isogenic clones stably expressing the MSCV-empty vector (T84-V) or the MSCV-MMP-9 vector (T84-MMP-9). Cells were treated in vitro (24 h in serum-free media) with PBS (upper panels) or ST (1 μM, lower panels). Bar, 200 μm. B, Tumor cell seeding of mouse lung in vivo was quantified as described in Methods. Results are percentages of respective vehicle-treated controls. Uroguanylin, 1 μM; 8-pCPT-cGMP, 1 mM; MMP-9, 500 ng/ml of purified human pro-MMP-9; BB94, a broad MMP-9 inhibitor (60 nmol/L). MMP-9 and BB94 were added to cell cultures during the last 2 h of incubations. With the exception of uroguanylin (N=2), at least 3 animals per condition were examined. *, P < 0.05; ***, P < 0.005 versus respective control.