Fig. 3.

JNK1 does not regulate HIF-1α expression. BMDM from either WT or Jnk1^−/− mice were cultured under normoxia (Po₂ = 21%) or hypoxia (Po₂ = 0.5%) for 4 h. (A) Cells were harvested, and cytosolic and nuclear extracts were prepared. Protein expression of JNK1/2 (cytosolic) and HIF-1α (nuclear) was examined by immunoblotting. (B) RNA was extracted and quantified by Q-RT-PCR. Results of three separate experiments done in triplicate are expressed as means ± SEM.