Fig. 2.
NT KO mice are protected from colonic tissue damage, adipose inflammation, and NF-κB activation caused by TNBS-induced colitis. Homozygous NT KO and wild-type mice were treated with TNBS for 2 days and body weight changes (A) and gross colitis score (B) were measured. ***P < 0.001, **P < 0.01 vs. wild-type, TNBS-treated mice. Mesenteric fat tissues from the same mouse groups were either stained with hematoxylin and eosin (C) or an antibody against phosphorylated p65 (D). (E) Mesenteric fat IL-6 levels of wild-type mice throughout the course of TNBS treatment were measured. ###P < 0.001 vs. day 0 of TNBS treated mice. (F) Mesenteric fat IL-6 levels on day 2 post-TNBS from wild-type and NT KO mice were measured. All experiments are representative of six mice per group. *** P < 0.001 vs. wild-type mice with TNBS treatment. Magnification ×100 (C) or ×200 (D).