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. 2009 May 7;106(21):8617–8622. doi: 10.1073/pnas.0900592106

Fig. 2.

Fig. 2.

The transcriptional level of jadJ and jadR1 with or without ethanol and the JadR1-protected regions upstream of jadJ and jadR1. (A) Determination of the tsps of jadJ and jadR1 by high-resolution S1 mapping of WT RNA (isolated at 48 h after ethanol treatment). (B) DNase I footprinting assays of JadR1 on the jadJ and jadR1 promoter regions. The concentrations (nM) of JadR1 are shown above the lanes. The lanes marked DNA are controls without protein. Each lane contains 200 ng of γ-32P-labeled DNA. The brackets denote the regions protected by JadR1, and the numbers on the right side indicate the distances relative to the respective tsps of jadJ and jadR1. (C and D) The transcriptional level of jadJ and jadR1 with or without ethanol induction was detected by low-resolution S1 analysis. Total RNAs from WT and jadR1DM in the presence (C) or absence (D) of ethanol were hybridized with jadJ and jadR1 probes. hrdB transcription was assayed as a control.