Fig. 4.
Effect of JdB and related molecules on the binding of JadR1 to its DNA targets. (A and B) Effect of JdB on the band-shift assay of JadR1 (50 nM) binding to PjadJ (A) and PjadR1 (B). (C and D) Dose-response curves of the inhibition by JdB of the binding of JadR1 (150 nM) to PjadJ (C) or PjadR1 (D). (E) Effect of JdB, JdA, DHU, and DHR on the DNA binding activity of JadR1. Band-shift assays were performed with 50 nM JadR1 and a range of ligand concentrations as indicated. The structures of compounds are present on the right side. Red arrows indicate the direction of biosynthesis, but the blue arrow points to a shunt product. (F) SPR analysis of the binding of JdB to immobilized JadR1. (G) SPR analysis of the binding of JdB to immobilized JadR1R (REC domain of JadR1).