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. 2009 May 7;106(21):8543–8548. doi: 10.1073/pnas.0809138106

Fig. 2.

Fig. 2.

Acid and thermal denaturation of WT TrAvrPto (WT) and H87Y-TrAvrPto (H87Y). (A) NMR peaks for a representative residue (Gly 95) showing the folded and unfolded peaks at the same contour level at different pH values. (B) Population of the folded state (pF) versus pH at 26.6 °C for the WT from NMR (filled diamonds), CD (open circles) Fl (open triangles) experiments and for H87Y from Fl (filled triangles) experiment. Solid lines, fitted curve for WT; dashed line, fitted by using WT pKa data without including COOH titration; dotted line, curve fitted to H87Y Fl data by using all WT pKa values except H87Y. (C) Thermal denaturation data for WT and H87Y. WT Fl at pH 4.0 (open circles), 4.7 (open squares), 5.4 (open diamonds), and 7.1 (open triangles). WT CD at pH 4.0 (filled circles), 4.6 (filled squares), 5.5 (filled diamonds), and 8.0 (filled triangles). H87Y Fl at pH: 5.3 (long-dashed line), 7.2 (short-dashed line). Vertical line marks 26.6 °C.