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. 2009 May 7;106(21):8647–8652. doi: 10.1073/pnas.0811584106

Fig. 4.

Fig. 4.

Attenuation of the T cell responses is not because of competition for peptide binding to MHC class II molecules. (A) To assess competition for peptide binding in vivo, BALB/c mice were immunized with 25 nmol of the low-stability variant MalE V1 in an emulsion containing IFA/PBS and 0.6 μg/mL LPS alone (○) or as a mixture with 5 nmol of the dominant peptides that bind I-Ad HEL V1 and MalE[102-115] (A, ●) or as a mixture with 5 nmol of the dominant peptides that bind I-Ed HA[107-120] and HEL[103-117] (■) in the hind footpad with increasing doses of the test peptides. (B) CD4 T cell responses after restimulation with 20 μM of competing dominant peptides. Draining LNs from individual mice were pooled, n = 2 mice per group. The results are presented as the total number of responding T cells and are an average of 2 independent experiments ± SEM. Results were analyzed with the 1-way Student's t test; ***, P < 0.0005.