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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Nat Struct Mol Biol. 2009 Mar 15;16(4):397–404. doi: 10.1038/nsmb.1572

Figure 5.

Figure 5

The effect of eIF2-Met-tRNAi on directed hydroxyl radical probing of 18S rRNA with BABE-Fe-eIF3j. Primer extension analysis of 18S rRNA cleaved by BABE-Fe-modified eIF3j. The sequencing lanes are indicated (C, T, A, and G). Other lanes include 40S subunits in the absence or presence of EDTA/Fe and mock-derivatized eIF3j (-cys+EDTA-Fe) in the absence (lane7) or presence of eIF2-Met-tRNAi (TC; lane 8). Lanes corresponding to eIF3j derivatized with BABE-Fe at the positions indicated in the absence (lanes 9, 11, 13, and 15), or presence of TC (lanes 10, 12, 14, and 16) are indicated. Nucleotide positions of cleavage sites in the 18S rRNA are indicated, and colored circles correspond to the components added in each reaction, as depicted in the cartoon.