Skip to main content
. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Mol Cancer Res. 2009 Apr;7(4):592–600. doi: 10.1158/1541-7786.MCR-08-0316

FIGURE 4.

FIGURE 4

Src is required for ErbB2 mediated STAT3 activation and p21Cip1 upregulation. A. ErbB2 overexpressing cells were treated with 5 μM control (PP3) or Src inhibitor (PP2). Cell lysates were collected and analyzed by Western blotting. B. 435.ErbB2 and SKBR3 cells were transfected with the p21Cip1 reporter plasmids wild type (pGL3-p21−2400) or SIE mutant (pGL3-p21−2400mSIE) and treated with 5 μM PP3 or PP2 for 24 hours. Cell lysates were collected and luciferase activities were measured and standardized by transfection efficiency using renilla values. Error bars represent S.D. C. Indicated cells were transfected with either vector control (Vec) or dominant negative Src mutant (pSRC-DN) for 24 hours. Lysates were analyzed by Western blotting. D. Cells were co-transfected with vector control or pSrc-DN plasmids and the pGL3-p21−2400 reporter plasmid. Luciferase activities were measured as in (C).