Skip to main content
. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Mol Cancer Res. 2009 Apr;7(4):592–600. doi: 10.1158/1541-7786.MCR-08-0316

FIGURE 5.

FIGURE 5

Inhibition of STAT3 sensitized ErbB2 overexpressing breast cancer cells to Taxol. A. 435.ErbB2 and SKBR3 cells were treated with 50 μM of STAT3 inhibitory peptide or control peptide for 24 hours. Nuclear extracts were then subjected to EMSA. Band shifts indicated a STAT3 protein:DNA complex. Oct-1 protein:DNA binding serves as an loading control. B. 435.ErbB2 and SKBR3 cells were treated with control (CP) or STAT3 inhibitor peptide (SP) (100 μM for 435.ErbB2 and 50 μM for SKBR3) for 12 hours. Cells were then treated with different concentrations of Taxol for 24 hours. Cell growth inhibition was determined by MTS assay. Error bars represent S.D. Inset: Representative Western blot for p21Cip1 and β-actin (loading control) from cells treated with either control (CP) or STAT3 inhibitor peptide (SP) treatment (** p = 0.004; * p = 0.016; t-test).