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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Mol Cancer Res. 2009 Apr;7(4):592–600. doi: 10.1158/1541-7786.MCR-08-0316

FIGURE 6.

FIGURE 6

Inhibition of Src kinase sensitized ErbB2 overexpressing breast cancer cells to Taxol. A. 435.ErbB2 cells were treated as indicated for 18 hours. Cell lysates were collected and analyzed by western blot analysis for the indicated antibodies. Numbers represent relative intensity of each band compared to the non-treated (NT) control. Total STAT3 was used as the loading control for both P-STAT3-Y705 and p21. B. 435.ErbB2 cells were treated with DMSO (control) or AZD0530 for 18 hours. Chromatin Immunoprecipitation (ChIP assay) for SIE p21Cip1 promoter sequence was performed using antibodies against histone and STAT3. IgG and Histone immunoprecipitation served as negative and positive controls, respectively. Input represents 10% of the total. C. 435.ErbB2 and SKBR3 cells were treated with DMSO only or AZD0530 (2 μM) for 12 hours. After 12 hours, cell media was replaced with medium containing either Taxol (50 nM) only, AZD0530 only or combination of Taxol and AZD0530. Cell growth inhibition was determined by MTS assay at 36 hours. Results were normalized to DMSO controls (** p = 0.002; * p = 0.013; t-test).