Figure 7. Effects of TTA-A2 on body weight, activity, and active wake.

(A) Diet-induced obese mice were weighed prior to daily dosing with vehicle (filled squares) or 10 mg/kg TTA-A2 (open squares). ^#P < 0.05, ^§P < 0.001 versus vehicle, 2-way ANOVA. (B) Starting and ending body weights from A. *P < 0.01, TTA-A2 versus vehicle; unpaired 2-tailed Student’s t test. (C) Locomotor activity of animals dosed with vehicle (filled squares) or 10 mg/kg TTA-A2 (open squares) in 30-minute epochs. Data are averaged such that each point represents the mean of 8 days from 8 animals (64 data points). Gray bars above denote significant differences (P < 0.05, Bonferroni post-hoc analysis). (D) Sprague-Dawley rats (n = 8) were implanted with telemetry monitors, recorded in a 7-day crossover dosing paradigm, and scored for sleep stage. Gray bars above denote significant differences (P < 0.05, mixed ANOVA analysis). Gray inverted triangles indicate time of dosing; solid bars represent lights-off periods. Data are mean ± SEM.