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. 2009 May 26;119(6):1595–1603. doi: 10.1172/JCI39060

Figure 7. Expression of the L368Q hDAT and P395L hDAT in transiently transfected HEK293 cells via immunoblotting analysis.

Figure 7

Cells were transiently transfected with wild-type, L368Q, and P395L hDAT and reagent only. In each panel, the same amount of protein was loaded in all lanes. Biotinylated (surface) protein and total lysate were probed with anti-DAT antibody for detection of the relative expression level of wild-type and mutant DAT protein. Anti–β-actin antibody showed the relative equivalent loading of total protein.