Figure 2. CEACAM6 expression in cancer cells and adenovirus infectivity.
(A) Expression of CEACAM6 RNA in adenovirus-sensitive and -insensitive pancreatic cell lines as assayed by qPCR. The A549 cell line was used as a calibrator to calculate the relative quantity (RQ) values. (B) Expression of CEACAM6 protein in adenovirus-sensitive and -insensitive pancreatic cell lines as detected by Western blot analysis. (C) Sensitivity of colon cancer cell lines to adenovirus. EC50 values were derived by MTS assay as described above. (D) qPCR analysis showing the expression of CEACAM6 RNA in colorectal cancer cell lines. The A549 cell line was used as a calibrator to calculate the relative quantity values. (E) Expression of adenovirus receptor CAR in human colorectal cancer cell lines as analyzed by FACS. (F) Expression of integrin αv in human colorectal cancer cell lines as analyzed by FACS. All experiments were repeated at least 3 times. *P < 0.05, ***P < 0.001.