(A) Agarose gel analysis and qRT-PCR of transcript expression of human pituitary, thyroid, PBL, and bone marrow RNAs amplified with native and novel primer sets. PCR-amplified products of both native and novel TSH were detected in pituitary RNA; however, only novel TSH was detected in thyroid and PBL RNA. Neither native nor novel TSH products were detected in BM RNA. (B) The TSH gene was expressed in the pituitary, the thyroid, and PBL, but not BM. (C) 18s gene expression levels were equivalent in all four samples.