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. 2009 Summer;8(2):140–146. doi: 10.1187/cbe.09-01-0003

Table 1.

Estimation of the molecular mass of the large subunit of Rubisco extracted from various plants, based on the Western blot analysis

Sample Migration distance (pixels) Calculated molecular mass (kDa)a % difference from Rubisco standard
Rubisco standard (from spinach) 2442 52.6
Young celery leaf 2442 52.6 0
Young celery petiole 2436 52.9 0.6
Mature celery leaf 2430 53.3 1.3
Mature celery petiole 2490 50.0 −4.9
Snap pea pod 2412 54.3 3.2
Onion leaf 2448 52.3 −0.6
Bean leaf 2406 54.7 4.0

a For comparison, the expected molecular mass of the large subunit mature chain of Rubisco from Arabidopsis thaliana on SDS-polyacrylamide gel electrophoresis (PAGE) is 52.7 kDa.