Table 1.
Sample | Migration distance (pixels) | Calculated molecular mass (kDa)a | % difference from Rubisco standard |
---|---|---|---|
Rubisco standard (from spinach) | 2442 | 52.6 | |
Young celery leaf | 2442 | 52.6 | 0 |
Young celery petiole | 2436 | 52.9 | 0.6 |
Mature celery leaf | 2430 | 53.3 | 1.3 |
Mature celery petiole | 2490 | 50.0 | −4.9 |
Snap pea pod | 2412 | 54.3 | 3.2 |
Onion leaf | 2448 | 52.3 | −0.6 |
Bean leaf | 2406 | 54.7 | 4.0 |
a For comparison, the expected molecular mass of the large subunit mature chain of Rubisco from Arabidopsis thaliana on SDS-polyacrylamide gel electrophoresis (PAGE) is 52.7 kDa.