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. 2009 May 15;6:46. doi: 10.1186/1742-4690-6-46

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Copyright © 2009 Ross et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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VSV-G pseudotyped HIV-1 is more stable over time in BeWo wild type cells (A) and in BeWo-CD4⁺cells (B) compared to BaL, HXB2 and envelope-deficient HIV-1 virions. Following viral exposure, the viral suspension was removed, and replaced with fresh medium; cell samples were collected at different time points. The amount of intracellular p24 antigen present in cells lysed immediately after viral exposure (time 0) was considered to be 100%, and all subsequent time points were normalised to time 0. The results are the mean of at least three independent experiments. Error bars indicate SEM.