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. 2009 May 15;8:28. doi: 10.1186/1476-4598-8-28

Figure 5.

Figure 5

siRNA gene silencing. (A) A fluorescent microscopy picture demonstrates intracellular localization of siRNA tagged with green fluorescent protein (GFP). Most GFP tagged siRNA was seen in the cytoplasm while the nuclei (N) lack green fluorescence. (B) Doxorubicin level was measured after P-glycoprotein siRNA treatment. P-glycoprotein siRNA treatment increased doxorubicin level at 0.1 and 1 μM in comparison to Non-Silencing control siRNA. (NS: Non-Silencing siRNA, PG: P-glycoprotein siRNA) (C) Immunoblotting demonstrates decreased expression of Bcl-2, Bcl-xL, XIAP and P-glycoprotein by siRNA. (-: carrier only, NS: Nonsilencing siRNA, PG: P-glycoprotein) (D) Targeting antiapoptotic gene (Bcl-2, Bcl-xL and XIAP) did not change mRNA expression of P-glycoprotein. Only P-glycoprotein siRNA treatment decreased mRNA expression of P-glycoprotein. mRNA expression of P-glycoprotein of each siRNA treatment was divided by mRNA expression of nonsilencing control. (NS: Nonsilencing siRNA, PG: P-glycoprotein).