Table 4.
Real-time PCR results obtained using the available bacterial strains
| PCR Resultb | |||||
|---|---|---|---|---|---|
| Bacterial straina | Reference ID | IACc | invA | prot6E | fliC |
| Salmonella Enteritidis | CVS-140/1 | + | + | - | |
| Salmonella Enteritidis | CVS-141/1–5 | + | + | - | |
| Salmonella Enteritidis | CVS-4054/1 | + | + | - | |
| Salmonella Enteritidis | CVS-4311/1 | + | + | - | |
| Salmonella Enteritidis | CVS-4325/4, 5 | + | + | - | |
| Salmonella Enteritidis | CVS-4421/1 | + | + | - | |
| Salmonella Enteritidis | CVS-4516/1 | + | + | - | |
| Salmonella Enteritidis | CVS-4532/1 | + | + | - | |
| Salmonella Enteritidis | CVS-4540/1 | + | + | - | |
| Salmonella Enteritidis | CVS-4666/1 | + | + | - | |
| Salmonella Enteritidis | CVS-4756/1 | + | + | - | |
| Salmonella Enteritidis | CVS-4807//1–3 | + | + | - | |
| Salmonella Enteritidis | CVS-4809/2 | + | + | - | |
| Salmonella Enteritidis | CVS-4980/1 | + | + | - | |
| Salmonella Enteritidis | CVS-5212/1 | + | + | - | |
| Salmonella Enteritidis | CVS-54/1 | + | + | - | |
| Salmonella Enteritidis | CVS-4792/1 | + | + | - | |
| Salmonella Enteritidis | CVS-4754/1 | + | + | - | |
| Salmonella Enteritidis | CVS-2553/4 | + | + | - | |
| Salmonella Typhimurium | CVS-3225//1–5 | + | - | + | |
| Salmonella Typhimurium | CVS-4074/1 | + | - | + | |
| Salmonella Typhimurium | CVS-4076/1 | + | - | + | |
| Salmonella Typhimurium | CVS-4255/1 | + | - | + | |
| Salmonella Typhimurium | CVS-4345/4, 5 | + | - | + | |
| Salmonella Typhimurium | CVS-4979/1 | + | - | + | |
| Salmonella Typhimurium | CVS-4981/1 | + | - | + | |
| Salmonella Typhimurium | CVS-5090/1 | + | - | + | |
| Salmonella Typhimurium | CVS-55/1 | + | - | + | |
| Salmonella Typhimurium | CVS-920/1–3 | + | - | + | |
| Salmonella Typhimurium | CVS-131/2 | + | - | + | |
| Salmonella Typhimurium | CVS-729/2 | + | - | + | |
| Salmonella Typhimurium | CVS-3794/1 | + | - | + | |
| Salmonella Typhimurium | CVS-3822/1 | + | - | + | |
| Salmonella Typhimurium | CVS-1421/1 | + | - | + | |
| Salmonella Typhimurium | 14028 | + | - | + | |
| Salmonella Enteritidis | 13076 | + | + | - | |
| Staphylococcus aureus | 1803 | - | - | - | |
| Staphylococcus aureus | 25923 | - | - | - | |
| Bacillus cereus | 7464 | - | - | - | |
| Bacillus cereus | 11145 | - | - | - | |
| Bacillus cereus | 11778 | - | - | - | |
| Bacillus subtilis | 110649 | - | - | - | |
| Enterobacter aerogenes | 13048 | - | - | - | |
| Enterococcus faecalis | 29212 | - | - | - | |
| Escherichia coli | 25922 | - | - | - | |
| Escherichia coli O157 | 35150 | - | - | - | |
| Listeria innocua | 11288 | - | - | - | |
| Listeria ivanovie | 11846 | - | - | - | |
| Listeria ivanovie | 19119 | - | - | - | |
| Listeria monocytogenes | 11994 | - | - | - | |
| Micrococcus luteus | 9341 | - | - | - | |
| Proteus vulgaris | 13315 | - | - | - | |
| Pseudomonas aeruginosa | 27853 | - | - | - | |
| Rhodococcus equi | 1621 | - | - | - | |
| Salmonella Bredeney | 1030/1 | + | - | - | |
| Salmonella Infantis | 1030/4 | + | - | - | |
| Salmonella Anatum | 1030/5 | + | - | - | |
| Salmonella Hadar | 1030/6 | + | - | - | |
| Salmonella Newport | 1030/7 | + | - | - | |
| Salmonella Typhimurium | 1030/10 | + | - | + | |
| Salmonella Virchow | 1030/11 | + | - | - | |
| Salmonella Enteritidis | 1030/17 | + | + | - | |
a The origin of these samples has been described in tables 1, 2 and 3.
b Detectable real-time PCR amplification signal is denoted by the symbol (+) which indicates CT values ≤ 45. Undetectable signal (CT values >45) is denoted by (-) and indicates the absence of template DNA.
c IAC is an artificial internal amplification control that does not correspond to any of the submitted sequences in the Genbank.