Table 1.
Regulated genes identified in the SSH libraries of thermally challenged Bathymodiolus thermophilus with significant database matches (only sequences with E-value above 0.005 are shown).
| Homolog (protein); Blastx value | Homolog species | Insert size (bp) | Accession number | SSH library |
| Cell cycle, DNA repair, protein regulation and transcription | ||||
| Elongation factor 1 alpha; 1e-14 | Mytilus galloprovincialis | 428 | GH196568 | forward |
| Elongation factor beta; 2e-05 | Strongylocentrotus purpuratus | 153 | GH196577 | forward |
| Elongation factor-2; 9e-89 | Hutchinsoniella macracantha | 577 | GH196570 | forward |
| S-cyclophilin; 2e-14 | Gallus gallus | 384 | GH196575 | forward |
| H3 histone; 4e-43 | Mus musculus | 538 | GH196576 | reverse |
| Myc homolog; 7e-10 | Crassostrea virginica | 704 | GH196563 | reverse |
| Respiratory chain | ||||
| Cytochrome c oxidase subunit II; 5e-32 | Lampsilis ornata | 424 | GH196573 | reverse |
| Cytochrome c oxidase subunit III; 1e-20 | Arbacia lixula | 260 | GH196584 | reverse |
| ATP synthase; 3e-21 | Bos taurus | 415 | GH196588 | reverse |
| Metabolism | ||||
| S-adenosylhomocysteine hydrolase 2e-60 | Branchiostoma belcheri tsingtaunese | 505 | GH196574 | forward |
| Cytosolic Lactate/malate dehydrogenase; 2e-14 | Caenorhabditis elegans | 421 | GH196590 | forward |
| Arginine kinase; | Octopus vulgaris | 135 | GH196579 | forward |
| Anhydrase carbonic 2; 2e-02 | Oncorhynchus mykiss | 114 | GH196567 | forward |
| Delta-5-desaturase; 4e-25 | Strongylocentrotus purpuratus | 195 | GH196582 | forward |
| Stress response and detoxication | ||||
| Glutathione peroxidase; 0.001 | Bombyx mori | 101 | GH196562 | forward |
| Heat Shock Protein 90; 4e-21 | Tetraodon nigroviridis | 156 | GH196561 | forward |
| Cell communication, membrane receptors, immune system | ||||
| Kalicludine 1; 3e-16 | Anemonia sulcata | 406 | GH196560 | reverse |
| Techylectin-5A; 8e-29 | Tachypleus tridentatus | 337 | GH196558 | reverse |
| Electron-transfer-flavoprotein; 4e-57 | Strongylocentrotus purpuratus | 535 | GH196557 | reverse |
| Itm1 protein, 4e-27 | Xenopus laevis | 179 | GH196556 | reverse |
| C1q-like adipose specific protein; 2e-07 | Salvelinus fontinalis | 570 | GH196594 | reverse |
| β-1,3-N-acetylglucosaminyltransferase 6; 5e-48 | Homo sapiens | 586 | GH196571 | reverse |
| Secreted protein, acidic, rich in cysteine SPARC; 2e-20 | Artemia franciscana | 523 | GH196580 | forward |
| Defensin; 1e-04 | Haliotis discus hannai | 105 | GH196559 | forward |
| Cytoskeleton production and maintenance | ||||
| α-2-tubulin; 4e-27 | Gecarcinus lateralis | 348 | GH196585 | forward |
| Actin; 1e-35 | Mytilus galloprovincialis | 541 | GH196578 | forward |
| Adhesive plaque matrix protein; 6e-06 | Mytilus galloprovincialis | 291 | GH196589 | forward |
| Foot protein 2; 1e-6 | Mytilus edulis | 108 | GH196581 | forward |
| Pedal retractor muscle myosin; 6e-50 | Mytilus galloprovincialis | 426 | GH196564 | forward |
| Hemicentin; fibulin 6; 2e-14 | Rattus norvegicus | 304 | GH196587 | reverse |
| Ribosomal proteins | ||||
| Ribosomal protein L3; 2e-55 | Argopecten irradians | 495 | GH196591 | forward |
| Ribosomal protein L4; 4e-80 | Latimeria chalumnae | 926 | GH196586 | forward |
| Ribosomal protein S14; 3e-04 | Rattus norvegicus | 256 | GH196555 | forward |
| Ribosomal protein S15; 1e-64 | Argopecten irradians | 465 | GH196566 | forward |
| Ribosomal protein S19; 2e-09 | Chlamys farreri | 362 | GH196569 | forward |
| Ribosomal protein S25; 1e-28 | Crassostrea gigas | 379 | GH196583 | forward |
| Ribosomal protein L15; 7e-16 | Siniperca kneri | 214 | GH196565 | reverse |
| QM protein; 7e-11 | Apis mellifera | 622 | GH196572 | reverse |
| Unknown function | ||||
| CG33171-PC, isoform C; 4e-07 | Drosophila melanogaster | 615 | GH196592 | reverse |
| Repeat organellar protein-related; 1e-05 | Plasmodium yoelii yoelii | 765 | GH196595 | reverse |
| Unidentified sequences | ||||
| 39 sequences | GH196596 to GH196632 | |||
Forward: genes over-expressed at 10°C versus 20°C;
Reverse: genes over-expressed at 20°C versus 10°C.