Table 3.

Segregation analysis of EST-SSR markers in a Silene cross.

Locus	Male × female	Genotype of progeny	Expected ratio	Observed ratio	X2
SL_eSSR01	A/B × A/B	A/A:A/B:B/B	1:2:1	60:5:17	108.31*
SL_eSSR02	A/B × A/B	A/A:A/B:B/B	1:2:1	17:33:33	9.65
SL_eSSR03	A/Ba × A/C	A/A:A/C:A/B:B/C	1:1:1:1	18:8:34:13	19.35*
SL_eSSR04	A/B × A/B	A/A:A/B:B/B	1:2:1	27:52:2	21.96*
SL_eSSR05	A/B × C/D	A/C:A/D:B/C:B/D	1:1:1:1	21:5:25:29	16.60*
SL_eSSR06	A/B × A/C	A/A:A/C:A/B:B/C	1:1:1:1	8:25:21:27	8.55
SL_eSSR07	A/B × A/B	A/A:A/B:B/B	1:2:1	17:43:21	0.70
SL_eSSR08	A/B × A/B	A/A:A/B:B/B	1:2:1	22:23:32	15.07*
SL_eSSR09	A/B × A/B	A/A:A/B:B/B	1:2:1	4:32:19	9.65
SL_eSSR10	A/B × A/B	A/A:A/B:B/B	1:2:1	19:43:21	0.20
SL_eSSR11	A/B × C/Da	A/C:A/D:B/C:B/D	1:1:1:1	21:13:28:21	5.43
SL_eSSR12	A/B × A/C	A/A:A/C:A/B:B/C	1:1:1:1	27:16:21:19	3.12
SL_eSSR13	A/B × A/C	A/A:A/C:A/B:B/C	1:1:1:1	26:13:22:18	4.70
SL_eSSR14	A/B × A/B	A/A:A/B:B/B	1:2:1	24:43:19	0.58
SL_eSSR16	A/B × A/B	A/A:A/B:B/B	1:2:1	16:45:17	1.87
SL_eSSR17	A/B × A/B	A/A:A/B:B/B	1:2:1	8:53:18	11.92
SL_eSSR20	A/B × A/B	A/A:A/B:B/B	1:2:1	15:28:15	0.06
SL_eSSR21	A/B × A/A	A/B × A/A	1:1	57:22	15.50*
SL_eSSR22	A/B × A/C	A/A:A/C:A/B:B/C	1:1:1:1	5:24:19:23	13.00
SL_eSSR24	A/B × A/A	A/B × A/A	1:1	47:11	24.27*
SL_eSSR25	A/B × C/D	A/C:A/D:B/C:B/D	1:1:1:1	21:19:20:23	0.42
SL_eSSR26	A/B × A/B	A/A:A/B:B/B	1:2:1	31:36:19	0.31
SL_eSSR27	A/B × A/A	A/B × A/A	1:1	41:21	6.45
SL_eSSR28	A/B × A/C	A/A:A/C:A/B:B/C	1:1:1:1	22:21:21:13	2.74
SL_eSSR29	A/B × A/C	A/A:A/C:A/B:B/C	1:1:1:1	22:23:21:18	0.66

*Significant deviation from expected Mendelian ratio after Bonferroni correction (P < 0.002)

^a indicates an inferred null allele

90 F2 offspring from a cross between Silene latifolia and S. dioica were genotyped and segregation ratios tested.