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Interactions between hsp90.2 mutant proteins and RAR1 or SGT1b does not correlate with phenotype. (A–C) β-Galactosidase assay quantification of the results of yeast 2-hybrid interaction measurements between HSP90.2 and mutant variants with RAR1 (A), SGT1a (B), or SGT1b (C). (D) HSP90.2 and mutant variants accumulate to equivalent levels in yeast as measured by Western blot. RAR1 interacts normally with SGT1a in this assay.
