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DT-specific IgG1/IgG2a ratios of individual mouse after M-TCI. Sera were collected on day 56, DT-specific IgG1 and IgG2a titers were measured by sandwich ELISA. IgG1/IgG2a ratios were calculated only using IgG2a responders. Data shown as mean + SD (n = 8, **p < 0.01 and ***p < 0.001, one-way ANOVA with Tukey posttest).
