mRNA expression of E-cadherin, Slug, Snail and Twist in esophageal adenocarcinoma. Real time RT-PCR was employed to examine the expression of E-cadherin, Slug, Snail and Twist in ten esophageal adenocarcinoma specimens (ADC1-ADC10) each compared to Barrett’s metaplastic tissue collected from the same resection specimen. Barrett’s metaplastic tissue control (BM), normalized to 1.0.