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Ex vivo pressure-mediated transfection of rat myocardium. (A) Transfection efficiency increased with increasing pressure and with prolonged incubation in hearts both perfused with and surrounded on epicardial and endocardial surfaces by FITC–ODN solution (80 μM). (B) Optimum transfection was achieved with both coronary perfusion and submersion in a bath of FITC–ODN solution, the former contributing more significantly to total delivery. *, n = 4.
