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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Exp Hematol. 2009 May;37(5):559–572. doi: 10.1016/j.exphem.2009.02.005

Table 2.

Intensity of the STAT-5 immunostaining (expressed as gray units) in the nuclear area of proerythroblasts derived in vitro from normal proerythroblasts stimulated with different GF-combinations.

Nuclear STAT-5 immunostaining
Treatments Minimum Intensity Maximal Intensity Average Intensity
Prol 1392° 2149° 1764.7±123.2°
Stv 1223* 1914* 1470.0±89.5*
EPO 1344° 2576*° 1799.5±172.8°
DXM 1439° 2250° 1833.1±130.6°
EPO + DXM 1285* 1865* 1524.0±77.1*

The intensity of the immunostaining was determined by analyzing thirty randomly selected nuclei per experimental point with the MetaMorph Imaging System (Molecular Devices, Sunnyvale, CA). The acquiring settings were maintained constant for all samples.

P values were calculated by Anova.

*

p< 0.001 with respect to the corresponding Prol cells

°

p< 0.01 with respect to the corresponding Stv cells