Fig. 3.
Images of cortical vasculature stained with FITC. (a) MIP along the z direction of a 250-μm-thick 3D stack with a 932 × 932 μm2 FOV. The data set consists of 26 frames acquired at 10-μm increments. (b) MIP along the z direction of a 400-μm-thick 3D stack with a 233 × 233 μm2 FOV. The lateral (x, y) position of the image is marked with the window in (a). The data set consists of 400 frames acquired at 1-μm increments. The same data set is presented as a volumetric image in (c) and two MIPs along the z direction of 50-μm-thick regions are presented at depths of 200 μm and 350 μm in (d) and (e), respectively (Media 1). Scale bar is 100 μm.
