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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Opt Express. 2008 Dec 8;16(25):20848–20863. doi: 10.1364/oe.16.020848

Fig. 6.

Fig. 6

Intravital micrographs of a mouse popliteal lymph node. The image (a) was obtained after intravital staining of mitochondria in lymph node cells though i.v.-injection of Rhodamine 6G (red) and labeling of the blood plasma with FITC (green). SHG signals from reticular collagen fibers are shown in blue (Media 3). Media 4 represents MIPs of five optical sections spaced 2.5 μm apart. This time-lapse recording highlights the cellular dynamics within the lymph node in relation to the structural components, such as collagen fibers and blood vessels. The image (b) shows the lymph node conduit system filled through subcutaneous injection of the low-molecular-weight protein lysozyme tagged with Alexa Fluor 633 (red) into the footpad drained by this lymph node (Media 5). The conduits accompany the collagen network (blue). Scale bars are 50 μm. The imaging depths are 100–150 μm (a) and 30–75 μm (b).