Peptide physicochemical properties statistics of nLC-MS/MS experiments. A, relative distributions of peptide mass ranges. B, relative distributions of peptide GRAVY scores. C, relative distributions of peptide pI values (acidic, pI < 5; basic, pI > 7; neutral (aliphatic), pI = 6.02; neutral (balanced charges), 7 > pI > 5, pI ≠ 6.02). D, fractions of neutral (aliphatic) peptides among transmembrane peptides. E, fractions of neutral (aliphatic) and low mass peptides among all identified peptides. F, fractions of low mass peptides within TM peptides. elas., elastase; tryp., trypsin.