Table I.
Search specificity overview of PM nLC-MALDI-MS/MS experiments performed throughout this work
Shown are the number of repetitive runs, search engine enzyme definitions used, identified peptides, false discovery rates against decoy database, identified proteins, BR protein hit number, matched BR peptides, and BR sequence coverage.
| Sample
|
||||||
|---|---|---|---|---|---|---|
| PM trypsin MALDI | PM trypsin MALDI | PM elastase MALDI | PM elastase MALDI | PM elastase MALDI | PM elastase MALDI | |
| No. of runs | 4 | 4 | 6 | 6 | 6 | 6 |
| Search specificity | KR | None | AVLISTG | AVLIST | AVLI | None |
| Identified peptides | 264 | 310 | 486 | 501 | 328 | 673 |
| False positive (%) | 10.6 | 6.2 | 5.1 | 5.7 | 9.4 | 3.3 |
| Identified proteins | 160/52 | 119/49 | 160/48 | 160/47 | 137/30 | 165/56 |
| BR protein hit no. | 20 | 1 | 1 | 1 | 1 | 1 |
| BR peptides | 4 | 39 | 86 | 86 | 50 | 120 |
| BR sequence coverage (%) | 18.5 | 40.6 | 81.8 | 88 | 73.1 | 75.1 |